Summary
Type: SPE
Application #: 301200
Catalog#: 730012 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 200mg, 50/pk
Detection:
Conditions: Column conditioning:
2 ml methanol, then 2 ml dist. water
Sample application:
Force or aspirate the sample through the column in 2 – 3 min, rinse with 2 ml
water / methanol / 8 M aqueous urea solution (1 : 2 : 2, v/v/v)
Column washing:
1) 2 ml methanol / water (1 : 1, v/v)
2) 1 ml 0.2 M hydrochloric acid
3) 1 ml methanol / water (1 : 1, v/v)
4) 1 ml 0.01 M sodium hydroxide solution
5) 3 ml methanol / water (1 : 1, v/v)
then centrifuge 10 min to remove the remaining liquid
Elution:
500 μl diethyl ether
Authors: Macherey-Nagel
Source: LC Department